He married Eliza Wood, daughter of Joseph Wood, on 23 April 1826.
Children of Commander Charles James Hope Johnstone and Eliza Wood.
Anne Williamina Hope Johnstone d. 14 Jan 1899. Mary Josephine Hope Johnstone d. 22 Dec 1903. Maj.-Gen. James Charles Hope Johnstone b. 11 Jun 1835, d. 6 Oct 1884